Wikimedia Commons

File:Chemotaxis-and-Binding-of-Pseudomonas-aeruginosa-to-Scratch-Wounded-Human-Cystic-Fibrosis-Airway-pone.0150109.s001.ogv

Chemotaxis-and-Binding-of-Pseudomonas-aeruginosa-to-Scratch-Wounded-Human-Cystic-Fibrosis-Airway-pone.0150109.s001.ogv (Ogg Theora video file, length 30 s, 720 × 240 pixels, 476 kbps, file size: 1.7 MB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary

edit
Description
English: PAO1wt-GFP swim towards CFBE41o- wound. Fura-2-loaded CFBE41o- cells grown on a coverslip were incubated in Ringer containing PAO1-GFP (2 MOI) and PI while confocal and DIC images were recorded (1 image/20 sec). During control conditions bacteria swam randomly above the surfaces of the epithelial cells. After 5 mins of incubation the epithelium was wounded with a needle, which scraped away a swath of cells. In addition, cells adjacent to the wound leaked fura-2 and took up PI into nuclei (red). PAO1wt-GFP migrated rapidly to the wounded region, followed by a slow return to random migration over the surface during the ensuing 25 mins (total movie = 30 mins). At the end of the experiment, some bacteria remained immobile near wounded cells. Movie typical of >10 recordings.
Date
Source S1 Movie from Schwarzer C, Fischer H, Machen T (2016). "Chemotaxis and Binding of Pseudomonas aeruginosa to Scratch-Wounded Human Cystic Fibrosis Airway Epithelial Cells". PLOS ONE. DOI:10.1371/journal.pone.0150109. PMID 27031335. PMC: 4816407.
Author Schwarzer C, Fischer H, Machen T
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 4.0 International license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current10:11, 13 October 201630 s, 720 × 240 (1.7 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

You cannot overwrite this file.

There are no pages that use this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 360P 166 kbps Completed 18:45, 21 August 2018 8.0 s
VP9 240P 62 kbps Completed 18:45, 21 August 2018 6.0 s
WebM 360P 512 kbps Completed 10:11, 13 October 2016 7.0 s
QuickTime 144p (MJPEG) 156 kbps Completed 14:48, 19 October 2024 1.0 s

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Chemotaxis-and-Binding-of-Pseudomonas-aeruginosa-to-Scratch-Wounded-Human-Cystic-Fibrosis-Airway-pone.0150109.s001.ogv&oldid=855460032"