File:Chlamydia-trachomatis-Intercepts-Golgi-Derived-Sphingolipids-through-a-Rab14-Mediated-Transport-pone.0014084.s009.ogv
Chlamydia-trachomatis-Intercepts-Golgi-Derived-Sphingolipids-through-a-Rab14-Mediated-Transport-pone.0014084.s009.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 32 s, 320 × 240 pixels, 247 kbps overall, file size: 953 KB)
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editDescriptionChlamydia-trachomatis-Intercepts-Golgi-Derived-Sphingolipids-through-a-Rab14-Mediated-Transport-pone.0014084.s009.ogv |
English: Rab14-labeled vesicular structures carry sphingolipids to the inclusion. HeLa cells overexpressing GFP-Rab14wt (green) were infected for 24 h with C. trachomatis serovar L2 (MOI 5). Then, cells were labeled for 40 minutes with BODIPY TR Ceramide (red) and chased for 30 minutes prior fixation. Hoescht stained bacterial DNA (blue). Image was reconstructed from consecutive z-sections assessed by confocal microscopy. Note intrainclusion vesicular structures labeled with Rab14wt (green) full of sphingolipids (red) in close contact with bacteria (blue). |
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Source | Video S2 from Capmany A, Damiani M (2010). "Chlamydia trachomatis Intercepts Golgi-Derived Sphingolipids through a Rab14-Mediated Transport Required for Bacterial Development and Replication". PLOS ONE. DOI:10.1371/journal.pone.0014084. PMID 21124879. PMC: 2989924. | ||
Author | Capmany A, Damiani M | ||
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current | 04:52, 17 November 2012 | 32 s, 320 × 240 (953 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Capmany A, Damiani M |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Rab14-labeled vesicular structures carry sphingolipids to the inclusion. HeLa cells overexpressing GFP-Rab14wt (green) were infected for 24 h with C. trachomatis serovar L2 (MOI 5). Then, cells were labeled for 40 minutes with BODIPY TR Ceramide (red) and chased for 30 minutes prior fixation. Hoescht stained bacterial DNA (blue). Image was reconstructed from consecutive z-sections assessed by confocal microscopy. Note intrainclusion vesicular structures labeled with Rab14wt (green) full of sphingolipids (red) in close contact with bacteria (blue). |
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Date and time of digitizing | 2010 |