English: Endoscopic Procedure for VX2 Fragments Implantation. Using a fine endoscopic needle, 0.5 ml of saline was first injected into the submucosal layer to elevate the mucosa layer. Then, a puncture was made using a large endoscopic needle (a revised needle) across the mucosa into the submucosal layer, and the success of the puncture was confirmed by further elevation of the mucosa when the saline was injected. Next, approximately 0.3 ml of saline containing four tumor pieces was injected into the submucosal layer. Finally, a second 0.3 ml portion of saline was injected to rinse the needles and collect the stuck pieces.