English: Coronin-1A-deficient (Coro-1A−/−) T cells present a lower frequency of SDF-1α-induced ruffling compared to control/wild-type (WT) T cells. Mesenteric LN T cells from WT and Coro-1A−/− mice were seeded onto poly-L-lysine coated glass coverslips in the presence of SDF1α/CXCL 12 (30 nM) and imaged by time-lapse video-microscopy for 10 min. The image sequences (intervals of 15 sec) are shown for two WT and two Coro-1A−/− T cells. These are representative images from two independent experiments; at least 40 cells were visualized and analyzed in each experiment. Initial images of the first series in Movie S1 correspond to those shown in the top panels of Figure S8.