File:Deficiency-of-Huntingtin-Has-Pleiotropic-Effects-in-the-Social-Amoeba-Dictyostelium-discoideum-pgen.1002052.s010.ogv
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Deficiency-of-Huntingtin-Has-Pleiotropic-Effects-in-the-Social-Amoeba-Dictyostelium-discoideum-pgen.1002052.s010.ogv (Ogg Theora video file, length 12 s, 480 × 384 pixels, 13.49 Mbps, file size: 19.94 MB)
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editDescriptionDeficiency-of-Huntingtin-Has-Pleiotropic-Effects-in-the-Social-Amoeba-Dictyostelium-discoideum-pgen.1002052.s010.ogv |
English: EGTA blocks calcium rescue of hd− early development. Dictyostelium hd− cells do not exhibit pulsatile cAMP signaling or chemotactic streaming into aggregation territories when incubated with 1 mM CaCl2 in the presence of 1 mM EGTA. Hd− cells failed to initiate pulsatile waves and consequently do not stream into aggregation centers, become rounded and can be seen randomly colliding. Cells were imaged using brightfield microscopy and images were captured every 15 seconds over a period of 8 hours. Video is representative of the time between 5 and 6 hours with a frame rate of 50 msec. |
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Source | Video S6 from Myre M, Lumsden A, Thompson M, Wasco W, MacDonald M, Gusella J (2011). "Deficiency of Huntingtin Has Pleiotropic Effects in the Social Amoeba Dictyostelium discoideum". PLOS Genetics. DOI:10.1371/journal.pgen.1002052. PMID 21552328. PMC: 3084204. | ||
Author | Myre M, Lumsden A, Thompson M, Wasco W, MacDonald M, Gusella J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:10, 14 November 2012 | 12 s, 480 × 384 (19.94 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Myre M, Lumsden A, Thompson M, Wasco W, MacDonald M, Gusella J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | EGTA blocks calcium rescue of hd? early development. Dictyostelium hd? cells do not exhibit pulsatile cAMP signaling or chemotactic streaming into aggregation territories when incubated with 1 mM CaCl2 in the presence of 1 mM EGTA. Hd? cells failed to initiate pulsatile waves and consequently do not stream into aggregation centers, become rounded and can be seen randomly colliding. Cells were imaged using brightfield microscopy and images were captured every 15 seconds over a period of 8 hours. Video is representative of the time between 5 and 6 hours with a frame rate of 50 msec. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011-04 |