File:Defined-Essential-8™-Medium-and-Vitronectin-Efficiently-Support-Scalable-Xeno-Free-Expansion-of-pone.0151264.s004.ogv

Defined-Essential-8™-Medium-and-Vitronectin-Efficiently-Support-Scalable-Xeno-Free-Expansion-of-pone.0151264.s004.ogv(Ogg Theora video file, length 15 s, 604 × 480 pixels, 1.22 Mbps, file size: 2.24 MB)

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English: Human iPSCs (Gibco hiPSC line) cultured in spinner flasks with E8 medium and VtnB retain their differentiation potential. Cardiomyocyte differentiation was performed using Life Technologies Cardiomyocytes Differentiation Kit by plating hiPS cells harvested from microcarriers in GP. Spontaneous contracting regions on GP were observed at day 10 of differentiation.
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Source S1 Video from Badenes S, Fernandes T, Cordeiro C, Boucher S, Kuninger D, Vemuri M, Diogo M, Cabral J (2016). "Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems". PLOS ONE. DOI:10.1371/journal.pone.0151264. PMID 27149065. PMC: 4801338.
Author Badenes S, Fernandes T, Cordeiro C, Boucher S, Kuninger D, Vemuri M, Diogo M, Cabral J
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Date/TimeThumbnailDimensionsUserComment
current09:51, 13 October 201615 s, 604 × 480 (2.24 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 647 kbps Completed 19:09, 23 August 2018 9.0 s
Streaming 480p (VP9) 647 kbps Completed 07:22, 2 February 2024 1.0 s
VP9 360P 273 kbps Completed 19:09, 23 August 2018 6.0 s
Streaming 360p (VP9) 273 kbps Completed 09:21, 6 February 2024 1.0 s
VP9 240P 98 kbps Completed 19:09, 23 August 2018 5.0 s
Streaming 240p (VP9) 98 kbps Completed 15:48, 16 December 2023 1.0 s
WebM 360P 505 kbps Completed 09:51, 13 October 2016 7.0 s
Streaming 144p (MJPEG) 444 kbps Completed 02:52, 3 November 2023 1.0 s

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