File:Dynamic-Filament-Formation-by-a-Divergent-Bacterial-Actin-Like-ParM-Protein-pone.0156944.s004.ogv
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Dynamic-Filament-Formation-by-a-Divergent-Bacterial-Actin-Like-ParM-Protein-pone.0156944.s004.ogv (Ogg Theora video file, length 4.8 s, 407 × 249 pixels, 204 kbps, file size: 120 KB)
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editDescriptionDynamic-Filament-Formation-by-a-Divergent-Bacterial-Actin-Like-ParM-Protein-pone.0156944.s004.ogv |
English: Photobleached regions of ParM-YFP polymers show rapid recovery in the presence of ParR and parC . E. coli cells expressing ParM-YFP in the presence of ParR and parC were grown to mid-logarithmic phase fluorescence microscopy was undertaken. Regions of interest were photobleached using five iterations of five laser lines (458, 477, 488, 514 and 561 nm), each at 100% power. Recovery of fluorescence of photobleached cells was monitored by imaging every 7.6 seconds for three minutes. Captured images were processed using ImageJ v1.48. |
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Source | S4 Movie from Brzoska A, Jensen S, Barton D, Davies D, Overall R, Skurray R, Firth N (2016). "Dynamic Filament Formation by a Divergent Bacterial Actin-Like ParM Protein". PLOS ONE. DOI:10.1371/journal.pone.0156944. PMID 27310470. PMC: 4911067. | ||
Author | Brzoska A, Jensen S, Barton D, Davies D, Overall R, Skurray R, Firth N | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 03:07, 21 July 2016 | 4.8 s, 407 × 249 (120 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Photobleached regions of ParM-YFP polymers show rapid recovery in the presence of ParR and parC . |
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Author | Brzoska A, Jensen S, Barton D, Davies D, Overall R, Skurray R, Firth N |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | E. coli cells expressing ParM-YFP in the presence of ParR and parC were grown to mid-logarithmic phase fluorescence microscopy was undertaken. Regions of interest were photobleached using five iterations of five laser lines (458, 477, 488, 514 and 561 nm), each at 100% power. Recovery of fluorescence of photobleached cells was monitored by imaging every 7.6 seconds for three minutes. Captured images were processed using ImageJ v1.48. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-06-16 |