English: Real time injection of labeled Lm during image acquisition in the spleen of CD11c-EYFP mice. 107 Bodipy-630 labeled Lm were injected i.v. into the retro-orbital plexus while acquiring intravital images in the spleen of CD11c-EYFP mice. CD11c-EYFP+ cells are shown in green, Bodipy-630 labeled Lm are shown in red. The time series is shown first as the entire field of a 14 µm z-stack collapsed to show all slices. The following time series is a 3D reconstruction of the same data set zoomed to a Lm and DC interaction. Next, 3D reconstructions of z-stack images are shown in rotation for 3 examples of Lm and DC pair. Z-stacks were taken at 1 µm steps during intravital imaging. Scale bar lengths are labeled in each series. Video corresponds with data from Figure 1.