English: Z-stack from confocal microscopy as rendered 3D-movie corresponding to figure 2, panel Bii. Measles virus cells in the respiratory tract of macaques 2 to 3 days after infection. Measles virus cells are shown green due to a genetic modification of enhanced green fluorescent protein (EGFP) in the virus. Cell nuclei are shown in blue with DAPI staining.

Entire Figure 2 description: Figure 2 Early rMVKSEGFP replication in the respiratory tract.

(A) Virus isolation performed from nose and throat swabs (left two panels), and from BAL cells (right panel). Each symbol represents an individual animal, bars indicate the geometric mean. Key: VI: virus isolation; d.p.i.: days post infection. (B) Live cell confocal microscopy performed on agarose-inflated lung slices from animals on 2 and 3 d.p.i. EGFP+ cells are shown in green, DAPI was used to counter stain nuclei (blue). Three images were collected, labeled i, ii and iii. Panels iia and iib show infected cells in one image from different orientations. Matching 3D-videos for (Bi, Bii and Biii) are available as supporting data.