File:Efficient-Conversion-of-Astrocytes-to-Functional-Midbrain-Dopaminergic-Neurons-Using-a-Single-pone.0028719.s002.ogv
Efficient-Conversion-of-Astrocytes-to-Functional-Midbrain-Dopaminergic-Neurons-Using-a-Single-pone.0028719.s002.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 18 s, 640 × 480 pixels, 177 kbps overall, file size: 390 KB)
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editDescriptionEfficient-Conversion-of-Astrocytes-to-Functional-Midbrain-Dopaminergic-Neurons-Using-a-Single-pone.0028719.s002.ogv |
English: Calcium oscillations in an astrocyte-derived dopaminergic neuron. The genetically-encoded calcium indicator GCaMP3 is expressed under the control of the neuron-specific MAP2 promoter. Fluorescence level increases with increased levels of intracellular calcium. Rhythmic oscillations of calcium levels are seen, consistent with the midbrain dopaminergic phenotype. |
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Source | Movie S1 from Addis R, Hsu F, Wright R, Dichter M, Coulter D, Gearhart J (2011). "Efficient Conversion of Astrocytes to Functional Midbrain Dopaminergic Neurons Using a Single Polycistronic Vector". PLOS ONE. DOI:10.1371/journal.pone.0028719. PMID 22174877. PMC: 3235158. | ||
Author | Addis R, Hsu F, Wright R, Dichter M, Coulter D, Gearhart J | ||
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current | 04:02, 14 November 2012 | 18 s, 640 × 480 (390 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Addis R, Hsu F, Wright R, Dichter M, Coulter D, Gearhart J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Calcium oscillations in an astrocyte-derived dopaminergic neuron. The genetically-encoded calcium indicator GCaMP3 is expressed under the control of the neuron-specific MAP2 promoter. Fluorescence level increases with increased levels of intracellular calcium. Rhythmic oscillations of calcium levels are seen, consistent with the midbrain dopaminergic phenotype. |
Short title | MA-iDN D19 GCaMP3 |
Software used | |
Date and time of digitizing | 2011 |
Language | English |