File:Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s007.ogv
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Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s007.ogv (Ogg Theora video file, length 14 s, 694 × 520 pixels, 399 kbps, file size: 666 KB)
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editDescriptionExperimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s007.ogv |
English: Time-lapse imaging of U87MG cells expressing HttQ103YFP-pIRES-mRFPu treated with PI 24 hours post-transfection. Cells were visualized using filters that detect YFP or RFP. Fluorescence emanating from the YFP and RFP channels was merged to create a movie which shows an increase of IB formation and UPS dysfunction. Images acquired every 10 minutes using a 10× objective for a total of 24 hours. The movie shows rapid and progressive accumulation of the mRFPu reporter protein (red colour) after the addition of PI. This coincides with the formation of IB at an earlier time point (30 hours). At the conclusion of the movie there are considerably more IB and cell death events as compared to untreated cells (videos S1 and S2). |
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Source | Video S4 from Tang M, Proctor C, Woulfe J, Gray D (2010). "Experimental and Computational Analysis of Polyglutamine-Mediated Cytotoxicity". PLOS Computational Biology. DOI:10.1371/journal.pcbi.1000944. PMID 20885783. PMC: 2944785. | ||
Author | Tang M, Proctor C, Woulfe J, Gray D | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:55, 17 November 2012 | 14 s, 694 × 520 (666 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Tang M, Proctor C, Woulfe J, Gray D |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010-09 |