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File:Externalized-decondensed-neutrophil-chromatin-occludes-pancreatic-ducts-and-drivespancreatitis-ncomms10973-s4.ogv

Externalized-decondensed-neutrophil-chromatin-occludes-pancreatic-ducts-and-drivespancreatitis-ncomms10973-s4.ogv ‎(Ogg Theora video file, length 15 s, 300 × 300 pixels, 383 kbps, file size: 696 KB)

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English: Supplementary Movie 3 Human neutrophils loaded with an intracellular pH dye (pHrodo), which

exhibits red fluorescence only at acidic pH, were cultured at 37°C / 5% CO2 in isotonic HBSS supplemented with 50 mM NaHCO3 in the presence of the non-cell permeant DNA probe Sytox Green. A neutrophil already displaying alkalinized cytoplasmic pH executed sudden DNA release, whereas an adjacent cell, which has still retained its neutral pH did not. A similar effect was

also observed in other cells of the cultures.
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Source Video file from Leppkes M, Maueröder C, Hirth S, Nowecki S, Günther C, Billmeier U, Paulus S, Biermann M, Munoz L, Hoffmann M, Wildner D, Croxford A, Waisman A, Mowen K, Jenne D, Krenn V, Mayerle J, Lerch M, Schett G, Wirtz S, Neurath M, Herrmann M, Becker C (2016). "Externalized decondensed neutrophil chromatin occludes pancreatic ducts and drives pancreatitis". Nature Communications. DOI:10.1038/ncomms10973. PMID 26964500. PMC: 4793047.
Author Leppkes M, Maueröder C, Hirth S, Nowecki S, Günther C, Billmeier U, Paulus S, Biermann M, Munoz L, Hoffmann M, Wildner D, Croxford A, Waisman A, Mowen K, Jenne D, Krenn V, Mayerle J, Lerch M, Schett G, Wirtz S, Neurath M, Herrmann M, Becker C
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Date/TimeThumbnailDimensionsUserComment
current18:50, 28 October 201615 s, 300 × 300 (696 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 46 kbps Completed 08:25, 26 August 2018 4.0 s
Streaming 240p (VP9) 46 kbps Completed 04:13, 21 December 2023 1.0 s
WebM 360P 78 kbps Completed 09:16, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 161 kbps Completed 19:15, 10 November 2023 1.0 s

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