File:Ezrin-Interacts-with-the-SARS-Coronavirus-Spike-Protein-and-Restrains-Infection-at-the-Entry-Stage-pone.0049566.s001.ogv
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editDescriptionEzrin-Interacts-with-the-SARS-Coronavirus-Spike-Protein-and-Restrains-Infection-at-the-Entry-Stage-pone.0049566.s001.ogv |
English: Ezrin accumulates at sites of entry of SARS-CoV S-pseudotyped lentiviral particles. Vero E6 cells stably expressing RFP-ezrinwt were seeded onto a microscopy culture dish and were inoculated with SARSpp harboring a GFP-tagged Vpr protein (SARSpp GFP-Vpr) on ice. Unbound particles were washed with cold medium. The culture dish was then placed in a 37°C 5% CO2 chamber of a Total Internal Reflection Fluorescence (TIRF) microscope for 30 minutes to allow for internalization of particles. After 30 minutes, time-lapse images were acquired to follow the movements of SARSpp GFP-Vpr particles during the internalization process at and beneath the plasma membrane of Vero E6 cells expressing RFP-ezrin. Fifty frames were acquired at 3 seconds intervals. Scale bar = 40 µm. |
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Source | Movie S1 from Millet J, Kien F, Cheung C, Siu Y, Chan W, Li H, Leung H, Jaume M, Bruzzone R, Malik Peiris J, Altmeyer R, Nal B (2012). "Ezrin Interacts with the SARS Coronavirus Spike Protein and Restrains Infection at the Entry Stage". PLOS ONE. DOI:10.1371/journal.pone.0049566. PMID 23185364. PMC: 3504146. | ||
Author | Millet J, Kien F, Cheung C, Siu Y, Chan W, Li H, Leung H, Jaume M, Bruzzone R, Malik Peiris J, Altmeyer R, Nal B | ||
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current | 04:25, 30 November 2012 | 8.0 s, 922 × 922 (432 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Millet J, Kien F, Cheung C, Siu Y, Chan W, Li H, Leung H, Jaume M, Bruzzone R, Malik Peiris J, Altmeyer R, Nal B |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |