English: □ Control; ▪ 0.5 mM Palmitate. (a) Insulin-suppression of G6pc mRNA was significantly inhibited in hepatocytes treated for 20 h with palmitate in vitro. This suppression was not affected by preincubation with 20 µM of a pseudosubstrate inhibitor of the aPKCs (Inh). (b) On the other hand, insulin-stimulated induction of Srebp1c mRNA was maintained in palmitate-treated cells. Induction could, however, be inhibited by addition of the aPKC inhibitor (Inh). Relative expression is relative to no insulin. Data are averages of real-time PCR results +/− std. error of the mean, n = 10. * indicates p<0.05 between groups.