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File:Fusion-between-Leishmania-amazonensis-and-Leishmania-major-Parasitophorous-Vacuoles-Live-Imaging-of-pntd.0000905.s003.ogv

Fusion-between-Leishmania-amazonensis-and-Leishmania-major-Parasitophorous-Vacuoles-Live-Imaging-of-pntd.0000905.s003.ogv ‎(Ogg Theora video file, length 48 s, 320 × 240 pixels, 68 kbps, file size: 399 KB)

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English: Fusion between L. amazonensis and L. major PVs recorded by multidimensional imaging of macrophages previously infected with L. amazonensis-WT for 48 hours and then superinfected with L. major-DsRed2 metacyclic-enriched promastigotes. (A) Leishmania major-DsRed2 promastigote (red), sheltered within tight PV, weakly stained with Lysotracker (green), fused with large, Lysotracker-positive, L. amazonensis-WT PV. (B) Lysotracker channel, showing changes in PV shape and remodeling at the end of process. (C) Promastigote DsRed2 channel, showing the transfer of promastigote by parasite posterior pole. (D) Image rotation for visualization of fusion process from the bottom of the sample. Time after L. major-DsRed2 promastigote addition is shown (h:min). Multidimensional images were constructed using Imaris blend filter. Bar = 10 µm.
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Source Video S2 from Real F, Mortara R, Rabinovitch M (2010). "Fusion between Leishmania amazonensis and Leishmania major Parasitophorous Vacuoles: Live Imaging of Coinfected Macrophages". PLOS Neglected Tropical Diseases. DOI:10.1371/journal.pntd.0000905. PMID 21151877. PMC: 2998430.
Author Real F, Mortara R, Rabinovitch M
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Date/TimeThumbnailDimensionsUserComment
current21:51, 30 October 201248 s, 320 × 240 (399 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 40 kbps Completed 08:34, 27 August 2018 8.0 s
Streaming 240p (VP9) 40 kbps Completed 21:50, 21 December 2023 1.0 s
WebM 360P 46 kbps Completed 11:12, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 419 kbps Completed 10:04, 13 November 2023 1.0 s

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