File:HAM-5-Functions-As-a-MAP-Kinase-Scaffold-during-Cell-Fusion-in-Neurospora-crassa-pgen.1004783.s012.ogv
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editDescriptionHAM-5-Functions-As-a-MAP-Kinase-Scaffold-during-Cell-Fusion-in-Neurospora-crassa-pgen.1004783.s012.ogv |
English: Oscillatory movement of HAM-5-GFP and MAK-2-mCherry to punta, to the hyphal tip and eventual site of contact in hyphae undergoing chemotropic interactions and fusion. A 60-minute movie compressed in 17 seconds of two hyphae undergoing chemotropic interactions and cell fusion. Oscillatory co-recruitment of HAM-5-GFP and MAK-2-mCherry to puncta, to hyphal tips undergoing chemotropic growth, to sites surrounding the septum, and to the site of contact is observed. MAK-2-mCherry is also observed in nuclei, but HAM-5-GFP is not. Alternating oscillation of both HAM-5-GFP and MAK-2-mCherry to puncta and the hyphal tip in the two homing hyphae is observed before fusion, but after fusion, the oscillation of HAM-5-GFP and MAK-2-mCherry to puncta and septa is observed simultaneously in both hyphae. The upper left panel is made of brightfields images, the lower left panel of GFP-fluorescent images, the lower right panel of mCherry-fluorescent images and the upper right panel is of merged GFP and mCherry fluorescent images. |
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Source | Movie S5 from Jonkers W, Leeder A, Ansong C, Wang Y, Yang F, Starr T, Camp D, Smith R, Glass N (2014). "HAM-5 Functions As a MAP Kinase Scaffold during Cell Fusion in Neurospora crassa". PLOS Genetics. DOI:10.1371/journal.pgen.1004783. PMID 25412208. PMC: 4238974. | |||
Author | Jonkers W, Leeder A, Ansong C, Wang Y, Yang F, Starr T, Camp D, Smith R, Glass N | |||
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current | 20:47, 10 December 2014 | 17 s, 1,024 × 1,024 (6.2 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Jonkers W, Leeder A, Ansong C, Wang Y, Yang F, Starr T, Camp D, Smith R, Glass N |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | Oscillatory movement of HAM-5-GFP and MAK-2-mCherry to punta, to the hyphal tip and eventual site of contact in hyphae undergoing chemotropic interactions and fusion. A 60-minute movie compressed in 17 seconds of two hyphae undergoing chemotropic interactions and cell fusion. Oscillatory co-recruitment of HAM-5-GFP and MAK-2-mCherry to puncta, to hyphal tips undergoing chemotropic growth, to sites surrounding the septum, and to the site of contact is observed. MAK-2-mCherry is also observed in nuclei, but HAM-5-GFP is not. Alternating oscillation of both HAM-5-GFP and MAK-2-mCherry to puncta and the hyphal tip in the two homing hyphae is observed before fusion, but after fusion, the oscillation of HAM-5-GFP and MAK-2-mCherry to puncta and septa is observed simultaneously in both hyphae. The upper left panel is made of brightfields images, the lower left panel of GFP-fluorescent images, the lower right panel of mCherry-fluorescent images and the upper right panel is of merged GFP and mCherry fluorescent images. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014-11 |