File:Identification-of-Candida-glabrata-Genes-Involved-in-pH-Modulation-and-Modification-of-the-pone.0096015.s002.ogv
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editDescriptionIdentification-of-Candida-glabrata-Genes-Involved-in-pH-Modulation-and-Modification-of-the-pone.0096015.s002.ogv |
English: C. glabrata replicates in LysoTracker negative compartments. RAW264.7 macrophages were stained with LysoTracker Red (diluted 1∶10.000 in cell culture medium) 1 h prior to infection and during co-incubation with viable C. glabrata cells. After infection (MOI of 4) time-lapse microscopy was started and pictures were taken every 5 min at a magnification of 630×. While the majority of yeasts resides in LysoTracker negative compartments and replicates, killing of yeast cells and subsequent LysoTracker acquisition can be observed within a few macrophages (red arrows 1–3). In macrophage 2, yeast replication in close proximity to a killed C. glabrata cell is shown. |
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Source | Video S1 from Kasper L, Seider K, Gerwien F, Allert S, Brunke S, Schwarzmuller T, Ames L, Zubiria-Barrera C, Mansour M, Becken U, Barz D, Vyas J, Reiling N, Haas A, Haynes K, Kuchler K, Hube B (2014). "Identification of Candida glabrata Genes Involved in pH Modulation and Modification of the Phagosomal Environment in Macrophages". PLOS ONE. DOI:10.1371/journal.pone.0096015. PMID 24789333. PMC: 3838353. | ||
Author | Kasper L, Seider K, Gerwien F, Allert S, Brunke S, Schwarzmuller T, Ames L, Zubiria-Barrera C, Mansour M, Becken U, Barz D, Vyas J, Reiling N, Haas A, Haynes K, Kuchler K, Hube B | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 14:44, 10 May 2014 | 8.5 s, 1,388 × 520 (3.73 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Kasper L, Seider K, Gerwien F, Allert S, Brunke S, Schwarzmuller T, Ames L, Zubiria-Barrera C, Mansour M, Becken U, Barz D, Vyas J, Reiling N, Haas A, Haynes K, Kuchler K, Hube B |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |