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File:Inhibition-of-PIKfyve-by-YM-201636-Dysregulates-Autophagy-and-Leads-to-Apoptosis-Independent-pone.0060152.s005.ogv

Inhibition-of-PIKfyve-by-YM-201636-Dysregulates-Autophagy-and-Leads-to-Apoptosis-Independent-pone.0060152.s005.ogv ‎(Ogg Theora video file, length 41 s, 300 × 226 pixels, 164 kbps, file size: 825 KB)

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English: Time-lapse imaging of primary hippocampal neurons –1 µM YM-201636. Cultured hippocampal neurons were grown for 2 days in a 24-well plate before imaging with an inverted Axio Observer microscope and AxioVision software (Zeiss). The neurons were contained in a 37°C, 5% CO2 chamber during time-lapse imaging. YM-201636 (1 µM) was added at 0 min (immediately following frame 1) and phase contrast images were subsequently taken every 10 min over 48 h.
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Source Movie S2 from Martin S, Harper C, May L, Coulson E, Meunier F, Osborne S (2013). "Inhibition of PIKfyve by YM-201636 Dysregulates Autophagy and Leads to Apoptosis-Independent Neuronal Cell Death". PLOS ONE. DOI:10.1371/journal.pone.0060152. PMC: 3609765.
Author Martin S, Harper C, May L, Coulson E, Meunier F, Osborne S
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Date/TimeThumbnailDimensionsUserComment
current02:20, 31 March 201341 s, 300 × 226 (825 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 78 kbps Completed 11:46, 5 December 2023 6.0 s
WebM 360P 78 kbps Completed 15:28, 30 November 2023 2.0 s
Streaming 144p (MJPEG) 273 kbps Completed 14:53, 15 November 2023 0.0 s

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