English: Additional file 5: Movie S1 Visualization of IGF-II-biot-QD655 bound to IR-B-GFP and endocytosed. HeLa cells expressing IR-B-GFP were labeled in vivo with 50 nM IGF-II-biot and 2 nM QD655, incubated at 37°C for 1 hour, treated for 5 min with 0.1 mM Na-glycine pH 3.0, NaCl 0.5 M and fixed in 3.7% PFA. Images were taken with a confocal microscope by acquisition of 64 z-slices with 16 μm step size. Deconvolution was performed with Huygens Scientific Volume Imaging Huygens Professional Version 3.6, applying the Quick maximum likelihood estimation algorithm. The movie shows tridimensional reconstruction using the surface renderer process observed from different angles.