File:Kupffer-Cells-Hasten-Resolution-of-Liver-Immunopathology-in-Mouse-Models-of-Viral-Hepatitis-ppat.1002061.s010.ogv
Kupffer-Cells-Hasten-Resolution-of-Liver-Immunopathology-in-Mouse-Models-of-Viral-Hepatitis-ppat.1002061.s010.ogv (Ogg Theora video file, length 30 s, 352 × 288 pixels, 342 kbps, file size: 1.24 MB)
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editDescriptionKupffer-Cells-Hasten-Resolution-of-Liver-Immunopathology-in-Mouse-Models-of-Viral-Hepatitis-ppat.1002061.s010.ogv |
English: HBV replication-competent transgenic mice were injected with NaCl and 3 days later subjected to liver intravital microscopy. The movie depicts lobular liver sinusoids in a representative mouse that was infused 5 minutes earlier with 5×107 CFSE-labeled HBV-specific effector CD8 T cells. Analysis of movies derived from six independent HBV replication-competent transgenic mice revealed a sticking fraction (defined as the percentage of total CD8 T cells that became firmly adherent for ≥30 s while passing a liver sinusoid within a 30 minutes observation period) of 29.2%±3.1%. Note that the sticking fraction of effector CD8 T cells that were specific for an irrelevant antigen (LCMV) and were infused into HBV replication-competent transgenic mice was detected as less than 5% (not shown). Scale bar represents 50 µm. |
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Source | Video S2 from Sitia G, Iannacone M, Aiolfi R, Isogawa M, van Rooijen N, Scozzesi C, Bianchi M, von Andrian U, Chisari F, Guidotti L (2011). "Kupffer Cells Hasten Resolution of Liver Immunopathology in Mouse Models of Viral Hepatitis". PLOS Pathogens. DOI:10.1371/journal.ppat.1002061. PMID 21655107. PMC: 3107209. | ||
Author | Sitia G, Iannacone M, Aiolfi R, Isogawa M, van Rooijen N, Scozzesi C, Bianchi M, von Andrian U, Chisari F, Guidotti L | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:14, 17 November 2012 | 30 s, 352 × 288 (1.24 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Sitia G, Iannacone M, Aiolfi R, Isogawa M, van Rooijen N, Scozzesi C, Bianchi M, von Andrian U, Chisari F, Guidotti L |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011-06 |