File:LKB1AMPK-and-PKA-Control-ABCB11-Trafficking-and-Polarization-in-Hepatocytes-pone.0091921.s008.ogv
LKB1AMPK-and-PKA-Control-ABCB11-Trafficking-and-Polarization-in-Hepatocytes-pone.0091921.s008.ogv (Ogg Theora video file, length 6.4 s, 480 × 480 pixels, 590 kbps, file size: 461 KB)
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editDescriptionLKB1AMPK-and-PKA-Control-ABCB11-Trafficking-and-Polarization-in-Hepatocytes-pone.0091921.s008.ogv |
English: Expression of YFP-tagged ABCB11 in cultured mouse hepatocytes. A collagen sandwich culture of mouse hepatocytes was transduced with adenovirus containing YFP-tagged ABCB11 three days after cell isolation and seeding. Stack of fluorescent confocal images were acquired on day 6. The three dimensional image reconstituted from the stack demonstrates high expression in the canalicular membrane of the transduced cell. Some ABCB11-YFP-containing vesicular structures can also be observed in the submembrane region. |
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Source | Video S2 from Homolya L, Fu D, Sengupta P, Jarnik M, Gillet J, Vitale-Cross L, Gutkind J, Lippincott-Schwartz J, Arias I (2014). "LKB1/AMPK and PKA Control ABCB11 Trafficking and Polarization in Hepatocytes". PLOS ONE. DOI:10.1371/journal.pone.0091921. PMID 24643070. PMC: 3958433. | |||
Author | Homolya L, Fu D, Sengupta P, Jarnik M, Gillet J, Vitale-Cross L, Gutkind J, Lippincott-Schwartz J, Arias I | |||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 09:03, 14 December 2014 | 6.4 s, 480 × 480 (461 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Homolya L, Fu D, Sengupta P, Jarnik M, Gillet J, Vitale-Cross L, Gutkind J, Lippincott-Schwartz J, Arias I |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | Expression of YFP-tagged ABCB11 in cultured mouse hepatocytes. A collagen sandwich culture of mouse hepatocytes was transduced with adenovirus containing YFP-tagged ABCB11 three days after cell isolation and seeding. Stack of fluorescent confocal images were acquired on day 6. The three dimensional image reconstituted from the stack demonstrates high expression in the canalicular membrane of the transduced cell. Some ABCB11-YFP-containing vesicular structures can also be observed in the submembrane region. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |