Wikimedia Commons

File:Light-Mediated-Kinetic-Control-Reveals-the-Temporal-Effect-of-the-RafMEKERK-Pathway-in-PC12-Cell-pone.0092917.s015.ogv

Light-Mediated-Kinetic-Control-Reveals-the-Temporal-Effect-of-the-RafMEKERK-Pathway-in-PC12-Cell-pone.0092917.s015.ogv ‎(Ogg Theora video file, length 25 s, 309 × 387 pixels, 150 kbps, file size: 459 KB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary

edit
Description
English: Light-induced membrane recruitment of Raf1 resulted in prolonged retainment of nuclear ERK2-GFP. NIH3T3 cells were triply transfected with CIBN-CaaX, CRY2PHR-mCherry-Raf1, and ERK2-GFP. An epi-fluorescence microscope (Leica DMI6000B) equipped with an oil-immersion 100× objective was used for image acquisition. The blue light (∼472 nm) filtered from a mercury lamp was used as the stimulation light, whose power density was adjusted to ∼1 W/cm2 at the sample plane. Pulsed blue light (100-ms pulse duration, 30-s interval) was used for stimulation and imaging. After each pulse of blue light stimulation, a green light pulse was applied to acquire the fluorescence image of CRY2PHR-mCherry-Raf1. The same field of view is shown in Fig. 3A.
Date
Source Movie S3 from Zhang K, Duan L, Ong Q, Lin Z, Varman P, Sung K, Cui B (2014). "Light-Mediated Kinetic Control Reveals the Temporal Effect of the Raf/MEK/ERK Pathway in PC12 Cell Neurite Outgrowth". PLOS ONE. DOI:10.1371/journal.pone.0092917. PMID 24667437. PMC: 3965503.
Author Zhang K, Duan L, Ong Q, Lin Z, Varman P, Sung K, Cui B
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 4.0 International license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current15:43, 29 March 201425 s, 309 × 387 (459 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

You cannot overwrite this file.

There are no pages that use this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 360P 37 kbps Completed 20:43, 1 September 2018 5.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 17 kbps Completed 20:43, 1 September 2018 3.0 s
Streaming 240p (VP9) 17 kbps Completed 02:34, 15 January 2024 1.0 s
WebM 360P 435 kbps Completed 15:43, 29 March 2014 7.0 s
Streaming 144p (MJPEG) 161 kbps Completed 20:31, 9 November 2023 1.0 s

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Light-Mediated-Kinetic-Control-Reveals-the-Temporal-Effect-of-the-RafMEKERK-Pathway-in-PC12-Cell-pone.0092917.s015.ogv&oldid=561724770"