English: 3-day long time-lapse of dendritic spines on several dendrites belonging to one neuron expressing PSD-95:EGFP. This video contains 150 frames (maximum intensity projection of 10 sections, 0.8 µm apart) collected at 30 min intervals (75 hours in total) starting 71 hours (∼3 days) after the beginning of the experiment. CCh (20 µM) was applied 27 hours (cycle 196 in video) into the video. Apart from slight contrast enhancement, the images are unprocessed. Image quality is substantially degraded, however, due to the compression involved in preparing the video. The black margins surrounding the image are due to the inter-frame alignment required to compensate for minor positioning inaccuracies of the motorized XY stage. Cycle number, running time and presence of CCh are indicated in left top corner. Same experiment as that shown in Figures 2A–E, 3.