File:Melanoma-cell-lysosome-secretory-burst-neutralizes-the-CTL-mediated-cytotoxicity-at-the-lytic-ncomms10823-s21.ogv
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Melanoma-cell-lysosome-secretory-burst-neutralizes-the-CTL-mediated-cytotoxicity-at-the-lytic-ncomms10823-s21.ogv (Ogg Theora video file, length 33 s, 320 × 240 pixels, 62 kbps, file size: 254 KB)
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editDescriptionMelanoma-cell-lysosome-secretory-burst-neutralizes-the-CTL-mediated-cytotoxicity-at-the-lytic-ncomms10823-s21.ogv |
English: Supplementary Movie 20 The movie shows a 3-D reconstruction of a conjugate formed between a CTL and a melanoma cell that was pretreated with monensin. Cells were conjugated for 5 minutes and stained for perforin and CD107a (blue). The 3-D reconstructed images were processed using the Imaris software to show in green the perforin co-localizing with CD107a (mostly found intracellularly in CTL only), and in red the perforin not colocalizing with CD107a (detected extracellularly). |
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Source | Video file from Khazen R, Müller S, Gaudenzio N, Espinosa E, Puissegur M, Valitutti S (2016). "Melanoma cell lysosome secretory burst neutralizes the CTL-mediated cytotoxicity at the lytic synapse". Nature Communications. DOI:10.1038/ncomms10823. PMID 26940455. PMC: 4785227. | ||
Author | Khazen R, Müller S, Gaudenzio N, Espinosa E, Puissegur M, Valitutti S | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:47, 28 October 2016 | 33 s, 320 × 240 (254 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 20 |
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Author | Khazen R, Müller S, Gaudenzio N, Espinosa E, Puissegur M, Valitutti S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | The movie shows a 3-D reconstruction of a conjugate formed between a CTL and a melanoma cell that was pretreated with monensin. Cells were conjugated for 5 minutes and stained for perforin and CD107a (blue). The 3-D reconstructed images were processed using the Imaris software to show in green the perforin co-localizing with CD107a (mostly found intracellularly in CTL only), and in red the perforin not colocalizing with CD107a (detected extracellularly). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-03-04 |