File:Microtubules-Depolymerization-Caused-by-the-CK1-Inhibitor-IC261-May-Be-Not-Mediated-by-CK1-Blockage-pone.0100090.s005.ogv
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editDescriptionMicrotubules-Depolymerization-Caused-by-the-CK1-Inhibitor-IC261-May-Be-Not-Mediated-by-CK1-Blockage-pone.0100090.s005.ogv |
English: Effect of IC261, nocodazole, taxol or taxol/IC261 in TGN morphology in NRK cells. NRK cells stably expressing the fusion protein TGN38-EGFP were cultured in a flow-through chamber and observed by time-resolved fluorescence microscopy. At time point “0 min” cell were treated with DMSO (0.1%), 50 µM IC261, 5 µM nocodazole or 10 µM taxol. Here exemplary cells are shown for the stated time points. The solvent DMSO and the treatment with taxol showed no effect on the TGN structure. IC261 as well as nocodazole treatment fragmented the tubular membrane structure of the TGN into vesicles distributed throughout the cell. Line 6–7: At time point “−10 min” K* cells were treated with 10 µM taxol and from time point “0 min” on with 10 µM taxol +50 µM IC261. Additional treatment with taxol could prevent the IC261 induced effects on the TGN. |
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Source | Movie S1 from Stoter M, Kruger M, Banting G, Henne-Bruns D, Knippschild U (2014). "Microtubules Depolymerization Caused by the CK1 Inhibitor IC261 May Be Not Mediated by CK1 Blockage". PLOS ONE. DOI:10.1371/journal.pone.0100090. PMID 24937750. PMC: 4061085. | ||
Author | Stoter M, Kruger M, Banting G, Henne-Bruns D, Knippschild U | ||
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current | 21:56, 11 July 2014 | 13 s, 1,206 × 604 (4.36 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Stoter M, Kruger M, Banting G, Henne-Bruns D, Knippschild U |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Effect of IC261, nocodazole, taxol or taxol/IC261 in TGN morphology in NRK cells. NRK cells stably expressing the fusion protein TGN38-EGFP were cultured in a flow-through chamber and observed by time-resolved fluorescence microscopy. At time point “0 min” cell were treated with DMSO (0.1%), 50 µM IC261, 5 µM nocodazole or 10 µM taxol. Here exemplary cells are shown for the stated time points. The solvent DMSO and the treatment with taxol showed no effect on the TGN structure. IC261 as well as nocodazole treatment fragmented the tubular membrane structure of the TGN into vesicles distributed throughout the cell. Line 6–7: At time point “−10 min” K* cells were treated with 10 µM taxol and from time point “0 min” on with 10 µM taxol +50 µM IC261. Additional treatment with taxol could prevent the IC261 induced effects on the TGN. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |