File:Mitosis-in-Neurons-Roughex-and-APCC-Maintain-Cell-Cycle-Exit-to-Prevent-Cytokinetic-and-Axonal-pgen.1003049.s003.ogv
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Mitosis-in-Neurons-Roughex-and-APCC-Maintain-Cell-Cycle-Exit-to-Prevent-Cytokinetic-and-Axonal-pgen.1003049.s003.ogv (Ogg Theora video file, length 5.1 s, 380 × 377 pixels, 69 kbps, file size: 43 KB)
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editDescriptionMitosis-in-Neurons-Roughex-and-APCC-Maintain-Cell-Cycle-Exit-to-Prevent-Cytokinetic-and-Axonal-pgen.1003049.s003.ogv |
English: Collapse and lysis of binucleated R8 after dissociation. Related to Figure 3. Live imaging of 10968-Gal4>GFP labeled R8 cells stained with DraQ5. Neural cells from trypsin-dissociated ruc discs in which nuclei initially appear separated tend to collapse to single round binucleate cell, and may then lyse. Such fusion is further evidence that pairs of nuclei do share the cytoplasm of the same cell. Based on confocal images of fixed tissue, we expected twice as many binucleated cells from rux eye discs. Both axon shearing during dissociation and collapse and lysis after dissociation may explain the shortfall. |
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Source | Video S1 from Ruggiero R, Kale A, Thomas B, Baker N (2012). "Mitosis in Neurons: Roughex and APC/C Maintain Cell Cycle Exit to Prevent Cytokinetic and Axonal Defects in Drosophila Photoreceptor Neurons". PLOS Genetics. DOI:10.1371/journal.pgen.1003049. PMID 23209426. PMC: 3510051. | ||
Author | Ruggiero R, Kale A, Thomas B, Baker N | ||
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current | 22:56, 5 June 2013 | 5.1 s, 380 × 377 (43 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Ruggiero R, Kale A, Thomas B, Baker N |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | Collapse and lysis of binucleated R8 after dissociation. Related to Figure 3. Live imaging of 10968-Gal4>GFP labeled R8 cells stained with DraQ5. Neural cells from trypsin-dissociated ruc discs in which nuclei initially appear separated tend to collapse to single round binucleate cell, and may then lyse. Such fusion is further evidence that pairs of nuclei do share the cytoplasm of the same cell. Based on confocal images of fixed tissue, we expected twice as many binucleated cells from rux eye discs. Both axon shearing during dissociation and collapse and lysis after dissociation may explain the shortfall. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-11 |