File:Multicellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s012.ogv
Multicellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s012.ogv (Ogg Theora video file, length 6.0 s, 640 × 480 pixels, 448 kbps, file size: 328 KB)
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editDescriptionMulticellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s012.ogv |
English: Live-cell microscopy of the merge between two identical P. mirabilis HI4320 swarms. Strain HI4320 expressing VipA::sfGFP under control of pBAD is observed merging with HI4320 expressing dsRED. The images were taken at the boundary between the red and green swarms as the two swarms merge. Equal penetration into the opposing swarm is observed between identical swarms expressing different colored fluors. Swarms were visualized on swarm agar containing 10 mM L-arabinose to induce expression of the VipA::sfGFP translational fusion. Single channel fluorescence, brightfield, and merged images are presented. Also see Movie S4. |
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Source | Movie S3 from Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H (2013). "Multicellular Bacteria Deploy the Type VI Secretion System to Preemptively Strike Neighboring Cells". PLOS Pathogens. DOI:10.1371/journal.ppat.1003608. PMC: 3764213. | ||
Author | Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 20:30, 14 September 2013 | 6.0 s, 640 × 480 (328 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Live-cell microscopy of the merge between two identical P. mirabilis HI4320 swarms. Strain HI4320 expressing VipA::sfGFP under control of pBAD is observed merging with HI4320 expressing dsRED. The images were taken at the boundary between the red and green swarms as the two swarms merge. Equal penetration into the opposing swarm is observed between identical swarms expressing different colored fluors. Swarms were visualized on swarm agar containing 10 mM L-arabinose to induce expression of the VipA::sfGFP translational fusion. Single channel fluorescence, brightfield, and merged images are presented. Also see Movie S4. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-09 |