File:Nicotinamide-is-an-endogenous-agonist-for-a-C.-elegans-TRPV-OSM-9-and-OCR-4-channel-ncomms13135-s2.ogv
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Nicotinamide-is-an-endogenous-agonist-for-a-C.-elegans-TRPV-OSM-9-and-OCR-4-channel-ncomms13135-s2.ogv (Ogg Theora video file, length 37 s, 256 × 256 pixels, 152 kbps, file size: 689 KB)
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editDescriptionNicotinamide-is-an-endogenous-agonist-for-a-C.-elegans-TRPV-OSM-9-and-OCR-4-channel-ncomms13135-s2.ogv |
English: Supplementary Movie 1 GCaMP6 fluorescence in chordotonal neurons. Continuous imaging of chordotonal neurons was performed to demonstrate behaviour in response to mechanical stimulation and NAM administration. Imaging begins with the neurons at rest. Alternating periods stimulation and rest were performed before administration of NAM, after which alternating periods of stimulation and rest were observed and labelled. Note the difference in fluorescence intensity upon stimulation in the absence of NAM, the increase in fluorescence upon NAM administration, and the lack of response to stimulation in the presence of NAM. Continuous imaging was performed at 20x magnification with a zoom factor of 5 on a Zeiss LSM 510 with LSM software 4.0 sp2. |
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Source | Video file from Upadhyay A, Pisupati A, Jegla T, Crook M, Mickolajczyk K, Shorey M, Rohan L, Billings K, Rolls M, Hancock W, Hanna-Rose W (2016). "Nicotinamide is an endogenous agonist for a C. elegans TRPV OSM-9 and OCR-4 channel". Nature Communications. DOI:10.1038/ncomms13135. PMID 27731314. PMC: 5064019. | ||
Author | Upadhyay A, Pisupati A, Jegla T, Crook M, Mickolajczyk K, Shorey M, Rohan L, Billings K, Rolls M, Hancock W, Hanna-Rose W | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 16:26, 29 October 2016 | 37 s, 256 × 256 (689 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 1 |
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Author | Upadhyay A, Pisupati A, Jegla T, Crook M, Mickolajczyk K, Shorey M, Rohan L, Billings K, Rolls M, Hancock W, Hanna-Rose W |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | GCaMP6 fluorescence in chordotonal neurons. Continuous imaging of chordotonal neurons was performed to demonstrate behaviour in response to mechanical stimulation and NAM administration. Imaging begins with the neurons at rest. Alternating periods stimulation and rest were performed before administration of NAM, after which alternating periods of stimulation and rest were observed and labelled. Note the difference in fluorescence intensity upon stimulation in the absence of NAM, the increase in fluorescence upon NAM administration, and the lack of response to stimulation in the presence of NAM. Continuous imaging was performed at 20x magnification with a zoom factor of 5 on a Zeiss LSM 510 with LSM software 4.0 sp2. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-10-12 |