File:Nucleocapsid-Promotes-Localization-of-HIV-1-Gag-to-Uropods-That-Participate-in-Virological-Synapses-ppat.1001167.s008.ogv
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Nucleocapsid-Promotes-Localization-of-HIV-1-Gag-to-Uropods-That-Participate-in-Virological-Synapses-ppat.1001167.s008.ogv (Ogg Theora video file, length 7.7 s, 325 × 310 pixels, 95 kbps, file size: 89 KB)
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editDescriptionNucleocapsid-Promotes-Localization-of-HIV-1-Gag-to-Uropods-That-Participate-in-Virological-Synapses-ppat.1001167.s008.ogv |
English: Cell surface patches containing Gag and a uropod marker laterally move to and accumulate at a forming uropod. Time lapse images of a Gag-YFP-expressing T cell during repolarization. Gag-YFP(green)-expressing P2 cells were immunostained for PSGL-1 (red) as described in Figure 6. Cells were then depolarized by incubation at 4°C for 30 min. Approximately 5 min after chamber coverslips containing depolarized cells were transferred to the microscope stage maintained at 37°C, acquisition of live cell images at 30-s intervals was begun and continued for 27 min. Note that the small patches migrate and coalesce to the large patch at the cell pole that eventually forms the uropod. |
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Source | Movie S3 from Llewellyn G, Hogue I, Grover J, Ono A (2010). "Nucleocapsid Promotes Localization of HIV-1 Gag to Uropods That Participate in Virological Synapses between T Cells". PLOS Pathogens. DOI:10.1371/journal.ppat.1001167. PMID 21060818. PMC: 2965768. | ||
Author | Llewellyn G, Hogue I, Grover J, Ono A | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:02, 17 November 2012 | 7.7 s, 325 × 310 (89 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Llewellyn G, Hogue I, Grover J, Ono A |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010-10 |