English: The Constriction of the TgCentrin2 Basal Compartment at a Late Stage of the Cell Cycle Can Be Induced by Calcium Ionophore A23187 A parasite expressing EGFP-TgCentrin2 that has started its cytokinesis. EGFP-TgCentrin2 has been recruited to the basal complexes of the daughters at this point (one of which is indicated by the arrow in the first frame). Upon A23187 treatment, the EGFP-TgCentrin2 labeling clearly constricts from ∼0.9 μm to ∼0.5 μm in less than 10 min. The experiment was conducted at room temperature and the interval between each image was ∼30 s. The total elapsed time for the video sequence is 10 min and 43 s. Note in some frames, such as time-points 0:02:37, 0:05:43, and 0:06:43, the upper daughter basal complex appears to be transiently elongated. This is an artifact caused by a small piece of fluorescent material that floated into the field of view and was transiently superimposed on the image of the basal complex in the projection of the 3D stack. This small object can be seen as a separate entity at time-points 0:04:07, 0:04:37, and 0:05:07. Insets: 2× magnification of the upper daughter basal complex indicated by the arrow in the first frame. All images in the video are maximum intensity projections of deconvolved 3D stacks.