File:PhOTO-Zebrafish-A-Transgenic-Resource-for-In-Vivo-Lineage-Tracing-during-Development-and-pone.0032888.s006.ogv
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editDescriptionPhOTO-Zebrafish-A-Transgenic-Resource-for-In-Vivo-Lineage-Tracing-during-Development-and-pone.0032888.s006.ogv |
English: Segmented Time-Lapse of PhOTO-N Development During Late Gastrulation. This video shows segmented data for each frame of the time-lapse summarized in Figure S2. The nuclei were segmented: the red and orange spheres represent the EVL nuclei and the white spheres represent the migrating epiblast nuclei. The membrane of the orange-labeled EVL nucleus was segmented and is highlighted in all three panels of the image in enhanced contrast blue. (A) Segmented and photoconverted H2B-Dendra2 data (red) as well as the segmented EVL membrane data (blue) with the bright field data (grayscale) from the time-lapse video. The developing head and optic primordia can be seen during the time-lapse. (B) Segmented and photoconverted H2B-Dendra2 data as well as the enhanced contrast segmented EVL membrane data along with the merged animal pole images as seen in Video S1D. (C) Isolated zoomed images of the segmented nuclei and membranes during the time-lapse. Scale bars are 50µm. |
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Source | Video S2 from Dempsey W, Fraser S, Pantazis P (2012). "PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration". PLOS ONE. DOI:10.1371/journal.pone.0032888. PMID 22431986. PMC: 3303793. | ||
Author | Dempsey W, Fraser S, Pantazis P | ||
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current | 21:14, 30 October 2012 | 1.7 s, 1,984 × 1,476 (1.5 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Dempsey W, Fraser S, Pantazis P |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |