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File:Pinpointing-retrovirus-entry-sites-in-cells-expressing-alternatively-spliced-receptor-isoforms-by-1742-4690-11-47-S11.ogv

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English: Movie 6. ASLV-A entry into an early endosome and concomitant acidification of endosomal lumen in TVA950 cell. ASLV-A pseudovirus co-labeled with Gag-mKate2 (red) and EcpH-ICAM (green) enters the early mKO-Rab5+ endosome (blue) in a TVA950 cell. Due to colocalization of three colors virus-endosome complex appears whitish (arrow). Soon after virus entry, the endosome becomes acidic, as evidenced by the loss of the EcpH-ICAM signal (purple particle), which in turn leads to ASLV-A content release into the cytosol (blue particle). The frame rate is slower around the very brief interval between acidification and fusion. The movie is played at 5 frames/sec (40x speed). For more information, see Figure 4B.
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Source Video file from Padilla-Parra S, Marin M, Kondo N, Melikyan G (2014). "Pinpointing retrovirus entry sites in cells expressing alternatively spliced receptor isoforms by single virus imaging". Retrovirology. DOI:10.1186/1742-4690-11-47. PMID 24935247. PMC: 4065388.
Author Padilla-Parra S, Marin M, Kondo N, Melikyan G
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