English: A Z-series showing L protein localization at viral inclusions surrounded by RNA. BSR-T7 cells were infected with rVSV at an MOI of 3 and exposed to nocodazole at 4 hpi. Following a 1h incubation, cells were transfected with BrUTP, fixed 40 minutes later and the viral RNA (green) and L (blue) visualized by confocal microscopy as described in methods. The video of a single representative cell shows combined Z-stacks (0.26 µm thickness) of images taken through the cell shown in Figure 6A.