File:Rac1-Dependent-Collective-Cell-Migration-Is-Required-for-Specification-of-the-Anterior-Posterior-pbio.1000442.s016.ogv

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English: The projections on the leading wild-type AVE cells are located basally. 3D reconstruction (Hex-GFP, green) from stacks of 150–200 optical sections (step size: 0.5 µm) was performed using Amira ResolveRT 4.1. The embryo outline (dotted line) was determined in Metamorph (Region outline tool) using bright field images. A single orthogonal plane of the embryo is shown in gray scale. The bounding box (orange lines) represents the volume analyzed in all the sections. The movie (from 0 to 130 degrees) includes 60 individual planes, rotated at 6 frames per second. The leading cells display a snail-like shape, with columnar cell bodies and basal projections.
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Source Video S4 from Migeotte I, Omelchenko T, Hall A, Anderson K (2010). "Rac1-Dependent Collective Cell Migration Is Required for Specification of the Anterior-Posterior Body Axis of the Mouse". PLOS Biology. DOI:10.1371/journal.pbio.1000442. PMID 20689803. PMC: 2914637.
Author Migeotte I, Omelchenko T, Hall A, Anderson K
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Date/TimeThumbnailDimensionsUserComment
current00:00, 19 October 20169.8 s, 1,744 × 1,456 (4.6 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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