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File:RsEGFP2-enables-fast-RESOLFT-nanoscopy-of-living-cells-elife00248fs003.jpg

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English: (A) Off-switching kinetics of rsEGFP and rsEGFP2 embedded in a PAA layer (pH ~6.5) determined at different intensities of the 491 nm off-switching light. Each curve is an average of 100 measurements. In each measurement the proteins were switched into the on-state for 40 µs with 405 nm light (2 kW/cm²) and subsequently the decay of fluorescence was recorded over time at the indicated 491 nm light intensities. (B) Dependence of the residual fluorescence (off-state fluorescence) in the ensemble off-state as a function of the off-switching light intensity. (C) Dependence of the residual fluorescence (off-state fluorescence) in the off-state of Vimentin-rsEGFP or Vimentin-rsEGFP2 in living Ptk2 cells as a function of the off-switching light intensity.
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Source Image file from Grotjohann T, Testa I, Reuss M, Brakemann T, Eggeling C, Hell S, Jakobs S (2012). "rsEGFP2 enables fast RESOLFT nanoscopy of living cells". eLife. DOI:10.7554/eLife.00248. PMID 23330067. PMC: 3534202.
Author Grotjohann T, Testa I, Reuss M, Brakemann T, Eggeling C, Hell S, Jakobs S
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current18:45, 16 October 2014Thumbnail for version as of 18:45, 16 October 2014582 × 586 (91 KB)Recitation-bot (talk | contribs)Automatic upload of media from: doi:10.7554/eLife.00248

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