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File:Separase-Is-Required-for-Homolog-and-Sister-Disjunction-during-Drosophila-melanogaster-Male-Meiosis-pgen.1005996.s017.ogv

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English: Normal biorientation of bivalents followed by separation failure during meiosis I after THR depletion. Time-lapse analysis of progression through the first meiotic division in spermatocytes expressing cid-EGFP and His2Av-mRFP. Moreover, spermatocyte-specific THR depletion was induced by transgenic RNAi (thr-RNAi). The movie starts at the nuclear envelope breakdown. In prometaphase I, up to 4 DNA masses, each with two green centromere dots, are recognizable. Bivalents align into a metaphase I plate indicating that spindles and kinetochores are functional. However, homologous chromosomes fail to separate during anaphase I. Precise genotype description is given in S1 Table. Image stacks with 30 focal planes spaced by 1 μm were acquired with a time interval of 1 min. Scale bar = 5 μm.
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Source S6 Movie from Blattner A, Chaurasia S, McKee B, Lehner C (2016). "Separase Is Required for Homolog and Sister Disjunction during Drosophila melanogaster Male Meiosis, but Not for Biorientation of Sister Centromeres". PLOS Genetics. DOI:10.1371/journal.pgen.1005996. PMID 27120695. PMC: 4847790.
Author Blattner A, Chaurasia S, McKee B, Lehner C
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current01:29, 18 October 20168.9 s, 1,648 × 880 (1.49 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 720P 422 kbps Completed 08:34, 19 October 2018 9.0 s
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Streaming 144p (MJPEG) 197 kbps Completed 07:08, 17 November 2023 1.0 s

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