File:Specific-Syndecan-1-Domains-Regulate-Mesenchymal-Tumor-Cell-Adhesion-Motility-and-Migration-pone.0014816.s002.ogv

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English: Videos S1-S10 for Figure 4. Random movement assay on the syndecan-1 overexpressing cells. The cells were seeded in a 12-well glass bottom culture plate and incubated at 37°C for 6 hours before transfer to a Leica DMIRE2 Inverted Laboratory Microscope for imaging. Cells were maintained during imaging in normal culture condition. Five observation fields were randomly selected and time-lapse imaging was performed every 15 minutes over 16 hours with a 10× dry objective. Thus a video of 65 photos for each single cell monitored was yielded and can be played using Quicktime player or Windows Media player software. A representative video of each transfectant was selected to show its random movement: Video S1, B6FS EGFP; Video S2, B6FS RMKKK/EGFP; Video S3, B6FS 77/EGFP; Video S4, B6FS 78/EGFP; Video S5, B6FS FL/EGFP. Video S6, STAV-AB EGFP; Video S7, STAV-AB RMKKK/EGFP; Video S8, STAV-AB 77/EGFP; Video S9, STAV-AB 78/EGFP; Video S10, STAV-AB FL/EGFP. The difference of motility between different transfectants can be seen by comparison of videos of respective transfectants.
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Source Video S1 from Zong F, Fthenou E, Mundt F, Szatmári T, Kovalszky I, Szilák L, Brodin D, Tzanakakis G, Hjerpe A, Dobra K (2011). "Specific Syndecan-1 Domains Regulate Mesenchymal Tumor Cell Adhesion, Motility and Migration". PLOS ONE. DOI:10.1371/journal.pone.0014816. PMID 21731601. PMC: 3121713.
Author Zong F, Fthenou E, Mundt F, Szatmári T, Kovalszky I, Szilák L, Brodin D, Tzanakakis G, Hjerpe A, Dobra K
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current19:11, 17 November 20128.1 s, 1,384 × 1,038 (6.41 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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