File:Strength-of-Neisseria-meningitidis-binding-to-endothelial-cells-requires-highly-ordered-CD147β2-ncomms15764-s2.ogv
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DescriptionStrength-of-Neisseria-meningitidis-binding-to-endothelial-cells-requires-highly-ordered-CD147β2-ncomms15764-s2.ogv |
English: Supplementary Movie 1 CD147 receptors organize as dense clusters at bacterial adhesion sites.2D-STORM reconstruction sequence of CD147 distribution within clusters assembled at bacterial adhesion sites (related to Fig. 6b). Control HBMECs cells infected for 1 hour with meningococci were fixed and stained for CD147 using an Alexa Fluor 647 conjugated secondary antibody to perform optical super-resolution microscopy technique, allowing single-molecule localisation (dSTORM). 10,000 frames were recorded for each acquisition and reconstructed using QuickPALM 1.1 ImageJ plug with the default settings. |
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Source | Video file from Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard S, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott M, Coureuil M, Morand P, Lafont F, Nassif X, Marullo S, Bourdoulous S (2017). "Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β2-adrenoceptor clusters assembled by alpha-actinin-4". Nature Communications. DOI:10.1038/ncomms15764. PMID 28569760. PMC: 5461506. | ||
Author | Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard S, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott M, Coureuil M, Morand P, Lafont F, Nassif X, Marullo S, Bourdoulous S | ||
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Short title | Supplementary Movie 1 |
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Author | Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard S, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott M, Coureuil M, Morand P, Lafont F, Nassif X, Marullo S, Bourdoulous S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | CD147 receptors organize as dense clusters at bacterial adhesion sites.2D-STORM reconstruction sequence of CD147 distribution within clusters assembled at bacterial adhesion sites (related to Fig. 6b). Control HBMECs cells infected for 1 hour with meningococci were fixed and stained for CD147 using an Alexa Fluor 647 conjugated secondary antibody to perform optical super-resolution microscopy technique, allowing single-molecule localisation (dSTORM). 10,000 frames were recorded for each acquisition and reconstructed using QuickPALM 1.1 ImageJ plug with the default settings. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2017-06-01 |