File:Structural-Requirements-for-PACSINSyndapin-Operation-during-Zebrafish-Embryonic-Notochord-pone.0008150.s009.ogv
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editDescriptionStructural-Requirements-for-PACSINSyndapin-Operation-during-Zebrafish-Embryonic-Notochord-pone.0008150.s009.ogv |
English: Defective mediolateral intercalation in mild pacsin3 morphant notochord. Embryos at the one-cell stage were injected with 100 pg mRNA encoding mcRFP for membrane expression and 7.5 ng pacsin3 MO. At 9.0 hpf they were dechorionated and mounted in E3 medium for time-lapse confocal imaging of the differentiating notochord. Images were collected at 1 min. intervals from 90% epiboly (∼9.5 hpf) for 60 min to the one-somite stage (∼10.5 hpf) using a Leica confocal microscope. The image stack was converted into QuickTime video at 10 frames/sec. |
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Source | Video S2 from Edeling M, Sanker S, Shima T, Umasankar P, Höning S, Kim H, Davidson L, Watkins S, Tsang M, Owen D, Traub L (2009). "Structural Requirements for PACSIN/Syndapin Operation during Zebrafish Embryonic Notochord Development". PLOS ONE. DOI:10.1371/journal.pone.0008150. PMID 19997509. PMC: 2780292. | ||
Author | Edeling M, Sanker S, Shima T, Umasankar P, Höning S, Kim H, Davidson L, Watkins S, Tsang M, Owen D, Traub L | ||
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current | 02:58, 17 November 2012 | 12 s, 1,280 × 720 (4.2 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Defective mediolateral intercalation in mild pacsin3 morphant notochord. Embryos at the one-cell stage were injected with 100 pg mRNA encoding mcRFP for membrane expression and 7.5 ng pacsin3 MO. At 9.0 hpf they were dechorionated and mounted in E3 medium for time-lapse confocal imaging of the differentiating notochord. Images were collected at 1 min. intervals from 90% epiboly (?9.5 hpf) for 60 min to the one-somite stage (?10.5 hpf) using a Leica confocal microscope. The image stack was converted into QuickTime video at 10 frames/sec. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |