File:Structure-and-Function-of-ABCG2-Rich-Extracellular-Vesicles-Mediating-Multidrug-Resistance-pone.0016007.s005.ogv
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editDescriptionStructure-and-Function-of-ABCG2-Rich-Extracellular-Vesicles-Mediating-Multidrug-Resistance-pone.0016007.s005.ogv |
English: Localization of ABCG2 and F-actin in MCF-7/MR cells. Cells were grown for 7 days, fixed with 4% formaldehyde and permeabilized using Triton-X-100. Then, cells were reacted with anti-ABCG2 antibody (BXP-21, green fluorescence) as well as with rhodamine phalloidin which binds to actin filaments (red fluorescence). Nuclei were stained with DAPI (blue fluorescence). Slides were analyzed by Z-stack sections creating 12 optical slices (0.5µm thick each) using a laser-scanning confocal microscope (Zeiss LSM 510 META) at ×630 magnification. The merged image of all obtained slices was animated into a video using LSM image browser software (Zeiss Inc.). |
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Source | Video S1 from Goler-Baron V, Assaraf Y (2011). "Structure and Function of ABCG2-Rich Extracellular Vesicles Mediating Multidrug Resistance". PLOS ONE. DOI:10.1371/journal.pone.0016007. PMID 21283667. PMC: 3025911. | ||
Author | Goler-Baron V, Assaraf Y | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 02:45, 14 November 2012 | 2.4 s, 1,024 × 1,024 (277 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Goler-Baron V, Assaraf Y |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |