File:Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv012.ogv
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Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv012.ogv (Ogg Theora video file, length 21 s, 320 × 256 pixels, 2.34 Mbps, file size: 5.89 MB)
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editDescriptionSurface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv012.ogv |
English: Cytochalasin D Treatment Does Not Affect Calcium Flux Stimulated by Antibody Crosslinking AD10 T cells treated with 10 μM cytochalasin D for 1 h at 37 °C were attached on poly-l-lysine–coated cover glasses in a flow chamber; 10 μg/ml biotinylated anti-Vβ3 monoclonal antibody, KJ25, was introduced and incubated for 10 min. A few T cells fluxed calcium at low levels during incubation. The antibody was then washed away, and 10 μg/ml streptavidin was introduced. Crosslinking by streptavidin led to high-level calcium flux in most of the cells (the last quarter of the video). Calcium levels are displayed in pseudocolor. The speed of the video is 50 times real time. |
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Source | Video S12 from Ma Z, Sharp K, Janmey P, Finkel T (2008). "Surface-Anchored Monomeric Agonist pMHCs Alone Trigger TCR with High Sensitivity". PLOS Biology. DOI:10.1371/journal.pbio.0060043. PMID 18303949. PMC: 2253636. | ||
Author | Ma Z, Sharp K, Janmey P, Finkel T | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:50, 16 November 2012 | 21 s, 320 × 256 (5.89 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Cytochalasin D Treatment Does Not Affect Calcium Flux Stimulated by Antibody Crosslinking |
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Author | Ma Z, Sharp K, Janmey P, Finkel T |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-02 |