File:Survival-advantages-conferred-to-colon-cancer-cells-by-E-selectin-induced-activation-of-the-PI3K-1471-2407-11-285-S2.ogv
Survival-advantages-conferred-to-colon-cancer-cells-by-E-selectin-induced-activation-of-the-PI3K-1471-2407-11-285-S2.ogv (Ogg Theora video file, length 1 min 30 s, 640 × 480 pixels, 1.04 Mbps, file size: 11.14 MB)
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editDescriptionSurvival-advantages-conferred-to-colon-cancer-cells-by-E-selectin-induced-activation-of-the-PI3K-1471-2407-11-285-S2.ogv |
English: An anti-DR3 blocking antibody impairs the adhesion of colon cancer cells to E-selectin-expressing endothelial cells in a laminar flow chamber. HUVECs were trypsinized and grown for 24 hrs on gelatin-coated slides. These endothelial cells were treated with 20 ng/ml IL-1β for 4 h to induce the expression of E-selectin. The endothelial cell cultures were then placed in a laminar flow chamber under a shear stress of 1 dyne/cm2. Live HT29 cells (2 × 106 per assay) stained with Calcein AM were treated with an anti-DR3 blocking antibody (B65) for 30 min prior to being introduced in the medium circulating over endothelial cells. Videos were taken directly using a camera mounted on a TE2000 fluorescence microscope at ×20 magnification to follow the adhesion of HT-29 cells to HUVECs. |
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Source | Porquet N, Poirier A, Houle F, Pin A, Gout S, Tremblay P, Paquet E, Klinck R, Auger F, Huot J (2011). "Survival advantages conferred to colon cancer cells by E-selectin-induced activation of the PI3K-NF?B survival axis downstream of Death receptor-3". BMC Cancer. DOI:10.1186/1471-2407-11-285. PMID 21722370. PMC: 3177907. | ||
Author | Porquet N, Poirier A, Houle F, Pin A, Gout S, Tremblay P, Paquet E, Klinck R, Auger F, Huot J | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 17:24, 17 September 2013 | 1 min 30 s, 640 × 480 (11.14 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 2 |
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Author | Porquet N, Poirier A, Houle F, Pin A, Gout S, Tremblay P, Paquet E, Klinck R, Auger F, Huot J |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |