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File:TOM1L1-drives-membrane-delivery-of-MT1-MMP-to-promote-ERBB2-induced-breast-cancer-cell-invasion-ncomms10765-s2.ogv

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English: Supplementary Movie 1 Dynamic of mCherry-MT1-MMP in sh-Ctrl, sh-TOM1L1 or rescued BT-474 cells. Live timelapse imaging of confocal orthogonal projections (X/Z) of BT-474 cells that express the indicated shRNAs and were transfected with mCherry-MT1-MMP and GFP or GFP-TOM1L1 or GFP-δGAT. Cells were plated on Oregon-Green 488-Gelatin coated glass bottom dishes. Note the global dynamic and appearance of MT1-MMP at the basal plasma membrane in control cells but not in TOM1L1-depleted cells. This dynamic is rescued by GFP-TOM1L1 but not by GFP-δGAT (Upper panel: filled arrows), correlating with gelatin degradation ability of the cells visualized with classical confocal imaging (X/Y) (Lower panel: outlined arrows). Dotted-lines show Z section used for orthogonal imaging. Movies were captured using a Leica 63x/1.4 Oil HCX PL APO CSCS objective on confocal microscope Leica SP5-SMD. Images were taken every 1.5 s during 1 min. Images were treated using 0.5 pixel-Median-Filter (ImageJ).
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Source Video file from Chevalier C, Collin G, Descamps S, Touaitahuata H, Simon V, Reymond N, Fernandez L, Milhiet P, Georget V, Urbach S, Lasorsa L, Orsetti B, Boissière-Michot F, Lopez-Crapez E, Theillet C, Roche S, Benistant C (2016). "TOM1L1 drives membrane delivery of MT1-MMP to promote ERBB2-induced breast cancer cell invasion". Nature Communications. DOI:10.1038/ncomms10765. PMID 26899482. PMC: 4764922.
Author Chevalier C, Collin G, Descamps S, Touaitahuata H, Simon V, Reymond N, Fernandez L, Milhiet P, Georget V, Urbach S, Lasorsa L, Orsetti B, Boissière-Michot F, Lopez-Crapez E, Theillet C, Roche S, Benistant C
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