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File:Targeting-CD28-CTLA-4-and-PD-L1-Costimulation-Differentially-Controls-Immune-Synapses-and-Function-pone.0083139.s001.ogv

Targeting-CD28-CTLA-4-and-PD-L1-Costimulation-Differentially-Controls-Immune-Synapses-and-Function-pone.0083139.s001.ogv ‎(Ogg Theora video file, length 20 s, 182 × 136 pixels, 159 kbps, file size: 389 KB)

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English: Human Teff form long lasting contacts with APCs, in control condition. Representative time-lapse video of human Teff cells stained with Fura-2AM (fluorescent calcium probe), incubated at 37°C with unstained APCs (human EBV-B lymphoblastoid cells). Cells were added on 0.001% poly-L-lysine coated Lab-Tek chambers and images were taken every 15 sec over 25 minutes. Non-activated Teff (green fluorescence) become red after increase of calcium concentration, thus demonstrating activation. Average contact-time, motility and velocity are shown in Fig. 3A, B, E, F and G. Calcium responses are shown in Fig. 4A and B. Time is shown in minutes.
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Source Movie S1 from Dilek N, Poirier N, Hulin P, Coulon F, Mary C, Ville S, Vie H, Clemenceau B, Blancho G, Vanhove B (2013). "Targeting CD28, CTLA-4 and PD-L1 Costimulation Differentially Controls Immune Synapses and Function of Human Regulatory and Conventional T-Cells". PLOS ONE. DOI:10.1371/journal.pone.0083139. PMC: 3871694.
Author Dilek N, Poirier N, Hulin P, Coulon F, Mary C, Ville S, Vie H, Clemenceau B, Blancho G, Vanhove B
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current01:56, 31 December 201320 s, 182 × 136 (389 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 89 kbps Completed 10:44, 17 December 2023 3.0 s
WebM 360P 95 kbps Completed 14:31, 2 December 2023 1.0 s
Streaming 144p (MJPEG) 855 kbps Completed 03:28, 9 November 2023 1.0 s

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