File:The-Association-of-Myosin-IB-with-Actin-Waves-in-Dictyostelium-Requires-Both-the-Plasma-Membrane-pone.0094306.s006.ogv
The-Association-of-Myosin-IB-with-Actin-Waves-in-Dictyostelium-Requires-Both-the-Plasma-Membrane-pone.0094306.s006.ogv (Ogg Theora video file, length 2.5 s, 714 × 301 pixels, 1.26 Mbps, file size: 386 KB)
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DescriptionThe-Association-of-Myosin-IB-with-Actin-Waves-in-Dictyostelium-Requires-Both-the-Plasma-Membrane-pone.0094306.s006.ogv |
English: Co-localization of N154A and F-actin in live cells; example of a circular wave formed in the middle of a cell. myoB−-cells expressing mRFP-lifeact and GFP-N154A mutant were starved for 30 min after which 1 µM latrunculin was added and images were recorded for 125 s (1 frame every 5 s). A circular wave formed in the middle of the cell, merged with a peripheral wave and eventually formed a small circular wave at the cell periphery. N154A co-localized with the F-actin wave at all stages. Fluorescence of N154A was weaker at the early stages of wave formation and gained strength with wave expansion. This video corresponds to Fig. S3. |
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Source | Movie S2 from Brzeska H, Pridham K, Chery G, Titus M, Korn E (2014). "The Association of Myosin IB with Actin Waves in Dictyostelium Requires Both the Plasma Membrane-Binding Site and Actin-Binding Region in the Myosin Tail". PLOS ONE. DOI:10.1371/journal.pone.0094306. PMID 24747353. PMC: 3991602. | |||
Author | Brzeska H, Pridham K, Chery G, Titus M, Korn E | |||
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current | 23:13, 10 December 2014 | 2.5 s, 714 × 301 (386 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Brzeska H, Pridham K, Chery G, Titus M, Korn E |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | Co-localization of N154A and F-actin in live cells; example of a circular wave formed in the middle of a cell. myoB−-cells expressing mRFP-lifeact and GFP-N154A mutant were starved for 30 min after which 1 µM latrunculin was added and images were recorded for 125 s (1 frame every 5 s). A circular wave formed in the middle of the cell, merged with a peripheral wave and eventually formed a small circular wave at the cell periphery. N154A co-localized with the F-actin wave at all stages. Fluorescence of N154A was weaker at the early stages of wave formation and gained strength with wave expansion. This video corresponds to Fig. S3. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |