File:The-Human-Polyoma-JC-Virus-Agnoprotein-Acts-as-a-Viroporin-ppat.1000801.s008.ogv
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The-Human-Polyoma-JC-Virus-Agnoprotein-Acts-as-a-Viroporin-ppat.1000801.s008.ogv (Ogg Theora video file, length 9.1 s, 180 × 110 pixels, 89 kbps, file size: 99 KB)
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editDescriptionThe-Human-Polyoma-JC-Virus-Agnoprotein-Acts-as-a-Viroporin-ppat.1000801.s008.ogv |
English: HeLa cells transfected with pERedNLS-Agno and FRET probe (YC3.60). Cells coexpressing pERedNLS-Agno (Video S1) or pERedNLS (Video S2) and YC3.60 were incubated for 72 h. Permeability to Ca2+ in agnoprotein-expressing (Video S1) or mock-transfected (Video S2) cells was evaluated by the change in FRET ratio induced by the addition of 5 mM CaCl2 to the extracellular medium 100 sec after the start of recording. Fluorescent images of CFP and FRET were recorded every 10 sec for 15 min. Ratio images of FRET/CFP were created to represent FRET efficiency. In the IMD mode shown here, eight colors from red to blue are used to represent the FRET/CFP ratio. The upper (red) and lower (blue) limits of the ratio range are 4.5 and 2.6, respectively. |
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Source | Video S1 from Suzuki T, Orba Y, Okada Y, Sunden Y, Kimura T, Tanaka S, Nagashima K, Hall W, Sawa H (2010). "The Human Polyoma JC Virus Agnoprotein Acts as a Viroporin". PLOS Pathogens. DOI:10.1371/journal.ppat.1000801. PMID 20300659. PMC: 2837404. | ||
Author | Suzuki T, Orba Y, Okada Y, Sunden Y, Kimura T, Tanaka S, Nagashima K, Hall W, Sawa H | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 13:31, 17 November 2012 | 9.1 s, 180 × 110 (99 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Suzuki T, Orba Y, Okada Y, Sunden Y, Kimura T, Tanaka S, Nagashima K, Hall W, Sawa H |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | HeLa cells transfected with pERedNLS-Agno and FRET probe (YC3.60). Cells coexpressing pERedNLS-Agno (Video S1) or pERedNLS (Video S2) and YC3.60 were incubated for 72 h. Permeability to Ca2+ in agnoprotein-expressing (Video S1) or mock-transfected (Video S2) cells was evaluated by the change in FRET ratio induced by the addition of 5 mM CaCl2 to the extracellular medium 100 sec after the start of recording. Fluorescent images of CFP and FRET were recorded every 10 sec for 15 min. Ratio images of FRET/CFP were created to represent FRET efficiency. In the IMD mode shown here, eight colors from red to blue are used to represent the FRET/CFP ratio. The upper (red) and lower (blue) limits of the ratio range are 4.5 and 2.6, respectively. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010-03 |