File:The-PDZ-Protein-Na+H+-Exchanger-Regulatory-Factor-1-(NHERF1)-Regulates-Planar-Cell-Polarity-and-pone.0153144.s003.ogv
The-PDZ-Protein-Na+H+-Exchanger-Regulatory-Factor-1-(NHERF1)-Regulates-Planar-Cell-Polarity-and-pone.0153144.s003.ogv (Ogg Theora video file, length 3 min 20 s, 404 × 138 pixels, 63 kbps, file size: 1.51 MB)
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DescriptionThe-PDZ-Protein-Na+H+-Exchanger-Regulatory-Factor-1-(NHERF1)-Regulates-Planar-Cell-Polarity-and-pone.0153144.s003.ogv |
English: Ciliary function was evaluated using brain sections exposing the third ventricle. Fresh sections were mounted on a microscope slide and immersed in MEM. An aliquot (2 μl) of a fluorescent bead suspension was added within the cavity of the ventricle and the movement of the beads was examined using a 20X water immersion objective mounted on an Olympus microscope. Images were acquired at 1 second intervals. The video shows the motion of the beads in sections obtained from wild-type and NHERF1 knockout animals. The panel labeled “wild-type + azide” shows the Brownian motion of the beads in a slide containing a wild-type section poisoned with azide to stop all ciliary motion. Refer to the legend of Fig 4. |
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Source | S1 Movie from Treat A, Wheeler D, Stolz D, Tsang M, Friedman P, Romero G (2016). "The PDZ Protein Na+/H+ Exchanger Regulatory Factor-1 (NHERF1) Regulates Planar Cell Polarity and Motile Cilia Organization". PLOS ONE. DOI:10.1371/journal.pone.0153144. PMID 27055101. PMC: 4824468. | ||
Author | Treat A, Wheeler D, Stolz D, Tsang M, Friedman P, Romero G | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 06:16, 13 October 2016 | 3 min 20 s, 404 × 138 (1.51 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Ciliary function was evaluated using brain sections exposing the third ventricle. |
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Author | Treat A, Wheeler D, Stolz D, Tsang M, Friedman P, Romero G |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Fresh sections were mounted on a microscope slide and immersed in MEM. An aliquot (2 μl) of a fluorescent bead suspension was added within the cavity of the ventricle and the movement of the beads was examined using a 20X water immersion objective mounted on an Olympus microscope. Images were acquired at 1 second intervals. The video shows the motion of the beads in sections obtained from wild-type and NHERF1 knockout animals. The panel labeled “wild-type + azide” shows the Brownian motion of the beads in a slide containing a wild-type section poisoned with azide to stop all ciliary motion. Refer to the legend of Fig 4. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-04-07 |