File:The-Role-of-Myelin-in-Theiler's-Virus-Persistence-in-the-Central-Nervous-System-ppat.0030023.sv005.ogv
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editDescriptionThe-Role-of-Myelin-in-Theiler's-Virus-Persistence-in-the-Central-Nervous-System-ppat.0030023.sv005.ogv |
English: Frozen Section of Optic Nerve of a Mouse Inoculated in the Vitreous Chamber with TMEV and Sacrificed 4 d Later The section was doubly stained for CNPase (green) and TMEV capsid antigen (red). Nuclei were stained blue with DAPI. The section was examined with a Zeiss Axioplan 2 microscope equipped with the Apotome device in order to obtain 0.7 μm thick optical sections. The video illustrates how optical section Z-stacks were used to assess colocalization of fluorescent markers. The video shows an example of infected CNPase-positive cell (oligodendrocyte). |
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Source | Video S5 from Roussarie J, Ruffié C, Brahic M (2007). "The Role of Myelin in Theiler's Virus Persistence in the Central Nervous System". PLOS Pathogens. DOI:10.1371/journal.ppat.0030023. PMID 17305428. PMC: 1797621. | ||
Author | Roussarie J, Ruffié C, Brahic M | ||
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current | 11:24, 17 November 2012 | 2.4 s, 1,385 × 1,030 (1.85 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Frozen Section of Optic Nerve of a Mouse Inoculated in the Vitreous Chamber with TMEV and Sacrificed 4 d Later |
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Author | |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | The section was doubly stained for CNPase (green) and TMEV capsid antigen (red). Nuclei were stained blue with DAPI. The section was examined with a Zeiss Axioplan 2 microscope equipped with the Apotome device in order to obtain 0.7 ?m thick optical sections. The video illustrates how optical section Z-stacks were used to assess colocalization of fluorescent markers. The video shows an example of infected CNPase-positive cell (oligodendrocyte). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2007-02 |