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File:The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s011.ogv

The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s011.ogv (Ogg Theora video file, length 12 s, 300 × 300 pixels, 449 kbps, file size: 669 KB)

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English: (Related to Fig. 3C). GFP-Shigella infected macrophages eventually undergo cell death. A 72 hpf mpeg1:G/U:mCherry larva was injected iv and in the mesenchyme near the injection site, with a sublethal dose of GFP-Shigella and live imaged every 2′12″ from 20 mpi (t = 0 on the movie) until 2h30pi (t = 2h12hpi on the movie) by confocal fluorescence microscopy. A mCherry+ macrophage harboring GFP-Shigella over time (red phagocyte with green bacteria, white arrow) rounds up (white arrow, t = 19h51mpi on the movie) and progressively undergoes cell death as suggested by the loss of the red label (white arrow, by t = 1h56hpi on the movie), presumably killed by the GFP-Shigella. Note in the bottom right corner of the entire sequence, neutrophils that have engulfed the GFP-Shigella kill them over time (diffuse and decreasing of the GFP intensity). The maximum intensity projection from 6 planes every 2 µm is shown. Scale bar, 10 µm.
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Source Video S5 from Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E (2013). "The Zebrafish as a New Model for the In Vivo Study of Shigella flexneri Interaction with Phagocytes and Bacterial Autophagy". PLOS Pathogens. DOI:10.1371/journal.ppat.1003588. PMC: 3764221.
Author Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E
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current20:36, 14 September 201312 s, 300 × 300 (669 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 147 kbps Completed 23:08, 21 October 2018 5.0 s
WebM 360P 247 kbps Completed 16:01, 2 December 2023 1.0 s
QuickTime 144p (MJPEG) 288 kbps Completed 15:38, 14 October 2024 1.0 s

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