English: (Related to Fig. 3E). Neutrophils engulf dying, infected cells. A sublethal dose of GFP-Shigella was injected in the blood and in the mesenchyme of a 72 hpf lyz:dsRed larva and live confocal fluorescence imaging was performed every 2′28″. The sequence shown here was acquired from 7 hpi (t = 0 on the movie) to 15 hpi (t = 9h06 on the movie). GFP-Shigella are able to infect other cell types (in this case a muscle fiber, area delimited by white line, decorated by green bacteria) and invade their cytoplasm, as suggested by the accumulation of GFP signal over time in this muscle fiber (see t = 1h19, t = 2h26, t = 4h08, t = 5h00 on the movie). By t = 5h49, the infected muscle fiber dies and is engulfed by dsRed+ neutrophils (red phagocytes, yellow arrows). By t = 6h34, another dying, infected muscle fiber (delimited by white line) is quickly engulfed by red neutrophils (yellow arrow). By t = 7h09, another infected, dying muscle fiber (delimitated by white line) is engulfed by red neutrophils (yellow arrow). Note that red neutrophils recruited to the site of infection efficiently engulf and clear GFP-Shigella over time in this sequence. A maximum intensity projection of 4 planes of 2 µm is shown. Scale bar, 10 µm.